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Showing 2 results for Rotavirus
Yousef Yahyapour, Rahim Savadkoohi, Karim-Allah Hajian, Somayyeh Jalilvand, Rasool Hamkar,
Volume 10, Issue 1 (3-2008)
Abstract
Background & Objective: Viral gastroenteritis is associated with significant cause of morbidity and is a common cause of hospitalization in worldwide. This study was performed to assess the role of rota, adeno and astroviruses in children presenting with acute diarrhea in the main Children's Medical Hospital in Babol-Iran. Materials & Methods: Stool specimens from 208 children suffering from diarrhea were tested for the presence of rota, adeno and astroviruses by ELISA test during Winter 2004 to Autumn 2005. Data were then analyzed by the Chi-square and Fisher exact test with SPSS-10 software. Results: Rota, adeno and astrovirus antigens were detected in 61.1%, 2.9% and 2.4% of patients. Infants between 6 and 12 months of age were most frequently affected with rotavirus (29.8%), (p<0.05). Rotavirus infection was significantly less frequent in summer and spring than winter and autumn (p<0.05). Fever and vomiting were present in 55.1% and 51.7% of children, respectively. There was not significant correlation between rotavirus infection and gender of patients. Conclusion: This study showed that rotavirus can be regarded as a major etiologic agent of winter acute diarrhea in children under 2-years old in Babol. Therefore, it is suggested to provide proper vaccination to immunize the children.
Ahmadi E, Soleimanjahi H, Teimoori A,
Volume 16, Issue 4 (12-2014)
Abstract
Background and Objective: Rotaviruses are the members of the Reoviridae family containing double-stranded RNA (dsRNA) genome which are the main cause of gastroinentritis particularly in children less than three years. This study was designed to evaluate the detection of rotavirus genome by new silver staining method using polyacrylamide gel electrophoresis (PAGE). Method: In this descriptive study, the samples were collected from infected MA-104 cell culture and the RNA electrophoresis was performed in 10% polyacrylamide slab gels after RNA extraction. Results: According to polyacrylamide gel electrophoresis and sensitive staining analysis, rotavirus RNA segments were divided into 4 groups and single-nucleotides differences were clearly detected rapidly. Conclusion: New silver staining method using polyacrylamide gel electrophoresis has the capacity to detect the rotavirus electeropherotype within a few minutes even in small DNA/RNA pieces up to 7 picograms.
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