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Background and Objective: Ciprofloxacin is effective against a wide range of bacteria, particularly Enterobacteriaceae. However, studies have shown that the resistance of Escherichia coli to ciprofloxacin is enhanced. Thyme is one of the medicinal plants whose essential oil has anti-microbial effects. This study was aimed to investigate the antimicrobial properties of local Thyme essential oil alone and in combination with ciprofloxacin on Escherichia coli mutant strain with intermediate resistance.
Methods: In this descriptive laboratory study, Thyme plants (10 samples) were collected from Lorestan Province, west of Iran during 2016. Theses samples belonged to T. eriocali species. Plant essential oil was extracted by distillation method with Clevenger equipment. The antimicrobial properties of local thyme were determined by measuring minimum inhibitory concentration (MIC) of it alone and in combination with ciprofloxacin using sequential dilution method (macrodilution and microdilution methods) in Escherichia coli strains. Minimum bactericidal concentration (MBC) was measured by cultivation method. The interaction between essential oil and ciprofloxacin was determined by calculation of fractional inhibitory concentration index (FICI).
Results: MIC of essential oil for wild type strain MG1655 and mutant strain RE6 was 8 and 10 µl/ml, respectively. MBC was equal to MIC. 0.4 µl/ml of essential oil decreased 45 fold the MIC of ciprofloxacin in mutant strain and produced synergistic interaction (FICI=0.06).
Conclusion: Thyme essential oil in concentration less than its MIC in combination with ciprofloxacin via synergistic interaction reduces antibiotic MIC and antibiotic resistance.

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Background and Objective: Childhood behavioral and emotional problems may be continus to puberty, even extend to elderly. This study was carried out to determine the role of family accommodation of child symptoms, parenting style and parental stress in prediction of anxiety disorder in children with 6 - 10 years old in Golestan province, north of Iran.

Methods: In this descriptive-analytic study 300 children with 6-10 years old and their mothers were selected by randomized cluster sampling method during 2017. The questionnaires of family accommodation scale-anxiety (FAS-A, 2013), parent stress index-sort form (PSI-SF, 2000), Bamerind parenting styles questionnaire 1973 and Spence child anxiety scale-parent form (SCAS-P, 1998) were completed by the mothers.

Results: Increasing family accommodation and parenting stress significantly correlated with increasing children anxiety (P<0.05). The authenticated childbearing style by parents significantly correlated with reducing the anxiety in children (P<0.05), but authoritarian parenting and permissive parenting by parents significantly correlated with increasing the anxiety in children (P<0.05). Authenticated childbearing style along with authoritative parenting style, parental stress and family support could predict 49% of children's anxiety (F=97.91, P<0.0001).

Conclusion: Authoritative and authoritarian parenting, parenting stress, family accommodation has an effective role in predicting children's anxiety.