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:: Volume 24, Issue 2 (7-2022) ::
J Gorgan Univ Med Sci 2022, 24(2): 89-96 Back to browse issues page
Isolation, Cloning, and Expression of Streptococcus dysgalactiae Subspecies Superantigen by SDS-PAGE in Psoriasis Patients
Kumarss Amini * 1, Ali Habbadi2
1- Associate Professor, Department of Microbiology, Faculty of Science, Saveh Branch, Islamic Azad University, Saveh, Iran. , dr_kumarss_amini@yahoo.com
2- M.Sc in Microbiology, Faculty of Science, Saveh Branch, Islamic Azad University, Saveh, Iran.
Abstract:   (3204 Views)
Background and Objective: Streptococci are Gram-positive and anaerobic bacteria that are isolated from different sources. These bacteria are capable of producing superantigens, toxins, and biologically-active substances and are therefore very important in the field of infectious disease. Streptococcal pyrogenic exotoxins A (speA) is produced by various bacteria, including Streptococcus dysgalactiae. This study aimed at the isolation, cloning, and production of speA from streptococci from the skin of psoriasis patients.
Methods: In this descriptive-laboratory study, samples were taken from 60 psoriasis patients. S. dysgalactiae isolated were identified by different tests. The speA gene was cloned by the TA cloning method using PTG-19 vector into the Escherichia coli X11 blue as host. Expression of the cloned gene in recombinant colonies was evaluated by SDS-PAGE.
Results: Screening of white recombinant colonies confirmed the presence of speA genes. Expression of the speA gene in E. coli X11 blue was confirmed by SDS-PAGE.
Conclusion: Streptococcus superantigens can be considered as a rich source of vaccine production for different infections caused by these bacteria. By utilizing different cloning hosts and investigating optimal production conditions, S. dysgalactiae could be a candidate for future studies on vaccine production.
 
Keywords: Streptococcus dysgalactiae [MeSH], Escherichia coli [MeSH], speA gene [MeSH], Cloning [MeSH], Superantigens [MeSH], SDS-PAGE
Article ID: Vol24-27
Full-Text [PDF 968 kb]   (14432 Downloads)    
Type of Study: Original Articles | Subject: Microbiology
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Amini K, Habbadi A. Isolation, Cloning, and Expression of Streptococcus dysgalactiae Subspecies Superantigen by SDS-PAGE in Psoriasis Patients. J Gorgan Univ Med Sci 2022; 24 (2) :89-96
URL: http://goums.ac.ir/journal/article-1-3894-en.html
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Volume 24, Issue 2 (7-2022) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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