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:: Volume 22, Issue 2 (6-2020) ::
J Gorgan Univ Med Sci 2020, 22(2): 18-25 Back to browse issues page
Effect of copper oxide nanoparticles on toxicity, enzymatic changes and tissue structure of rat liver
Razieh Karshenas1 , Ali Noori * 2, Fatemeh Shahbazi3
1- M.Sc in Animal Physiology, Payame Noor University of Tehran, Iran
2- Assistant Professor, Department of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran , ali.noori55@gmail.com
3- Assistant Professor, Department of Biology, Payame Noor University, Iran
Abstract:   (10408 Views)
Background and Objective: Copper oxide nanoparticles, in addition to useful applications, may have adverse effects on the organisms.This study was done to determine the effect of copper oxide nanoparticles on liver toxicity, enzymes changes and liver histological structure of rats.
Methods: In this experimental study, 40 Wistar male rats were randomly allocated into 4 groups. During 10 days, five times (one day interval), 3 groups of rats were received 10, 20 and 30 mg/kg of copper oxide nanoparticles with a diameter of less than 50 nm and purity of 99% and a surface of 80 m2/g intraperitoneally, respectively. One group was considered as the control group. Activity of Lactate dehydrogenase (LDH), Alkaline phosphatase (ALP), Aspartate transaminase (AST) and Alanine aminotransferase (ALT) enzymes were tested in two stages (one day and 15 days after treatment). Also, liver tissue sections were prepared and stained with hematoxylin-eosin.
Results: No significant alterations of AST enzyme activity were not seen between different groups in two stages. The activity of ALT, ALP, and LDH enzymes in the first stage showed a significant increase in all treatment groups compared to control and returned to normal after 15 days. Rat's weight changes were not statistically significant between different groups. Histological studies revealed multiple tissue injuries in dose-dependent in treatment groups which included mild and severe hyperemia, hepatocytes degeneration, hyperplasia and inflammation.
Conclusion: Injection of low doses of copper oxide nanoparticles, after 15 days, although changes in enzyme activity return to normal, but significant disturbances observes in the structure of the liver tissue.
Keywords: Liver [MeSH], Copper oxide nanoparticles [MeSH], Toxicity [MeSH]
Article ID: Vol22-18
Full-Text [PDF 460 kb]   (17129 Downloads)    
Type of Study: Original Articles | Subject: Physiology - Pharmacology
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Karshenas R, Noori A, Shahbazi F. Effect of copper oxide nanoparticles on toxicity, enzymatic changes and tissue structure of rat liver. J Gorgan Univ Med Sci 2020; 22 (2) :18-25
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Volume 22, Issue 2 (6-2020) Back to browse issues page
مجله دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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