Glycoconjugates distribution in the lateral walls of spinal cord during mouse morphogenesis using lectin histochemical method

Vojoudi E,; Ebrahimi V,; Ebrahimzadeh Bideskan AR ,; Fazel AR ,

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Volume 18, Issue 1 (3-2016)

J Gorgan Univ Med Sci 2016, 18(1): 16-22

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Glycoconjugates distribution in the lateral walls of spinal cord during mouse morphogenesis using lectin histochemical method

Vojoudi E¹

, Ebrahimi V²

, Ebrahimzadeh Bideskan AR³

, Fazel AR ^*

⁴

1- Ph.D Candidate in Tissue Engineering, Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
2- Ph.D Candidate in Anatomical Sciences Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3- Associate Professor, Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
4- Professor, Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran , fazela@mums.ac.ir

Abstract: (11097 Views)

Background and Objective: Different organizers are involved in spinal cord development and differentiation by sending various messages. Specific glycoconjugates secreted from the cells of lateral wall of spinal cord can also act as neurogenesis and neural differentiation messengers. This study was carried out to determine the distribution of sugar compounds in the lateral walls of spinal cord during mice morphogenesis using lectin histochemistry method. Methods: In this experimental study, sections of BALB/c mice from 10-16 embryonic days were fixed in formalin and then histological sections were prepared. Tissue samples for reaction to the glycoconjugates were incubated with DBA, OFA, GSA1B4 and MPA lectins. Alcian blue with pH equal 2.5 was used for background staining. Results: DBA lectin did not react with the lateral wall of the spinal cord. MPA lectin showed severe reaction but consistent, especially in nerve fibers of the lateral wall of spinal cord. GSA1B4 lectin showed weak reaction in the cells and nerve fibers of the spinal cord, but severe reaction was clearly observed in blood vessels. OFA lectin showed severe reaction with α-L-Fucose terminal sugar in the lateral walls of the spinal cord in early stages of morphogenesis. Conclusion: The most reaction in the lateral walls of the spinal cord was related to OFA, which reflects the importance of fucose terminal sugar by connecting (1→6) to the penultimate sugar N-acetyl-D-glocosamin (Glc-Nac) in the development of spinal cord. Due to severe reaction of GSA1B4 to blood vessels of spinal cord, use of this lectin for vascular studies, is recommended.

Keywords: Development, Spinal Cord, Lectin, Histochemistry, OFA Lectin, Mouse

Full-Text [PDF 332 kb] [English Abstract] (19013 Downloads) | | Abstract (HTML) (420 Views)

Type of Study: Original Articles | Subject: Embryology

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Vojoudi E, Ebrahimi V, Ebrahimzadeh Bideskan AR, Fazel AR. Glycoconjugates distribution in the lateral walls of spinal cord during mouse morphogenesis using lectin histochemical method. J Gorgan Univ Med Sci 2016; 18 (1) :16-22
URL: http://goums.ac.ir/journal/article-1-2650-en.html

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